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Welcome to Yuzaki Lab
    Yuzaki Lab's (Department of Neurophysiology, Keio University School of Medicine) research theme is to elucidate "how neural activity and environmental changes induce memory and learning,、, 、and how they change the neural network itself ."。For details please Click here.
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2020

■ The amount of MeCP2 protein controls the three-dimensional structure of chromatin in nerve cells（J Neurosci）2020.10.12

Ito-Ishida A, Baker SA, Sillitoe RV, Sun Y, Zhou J, Ono Y, Iwakiri J, Yuzaki M, Zoghbi HY. MeCP2 Levels Regulate the 3D Structure of Heterochromatic Foci in Mouse Neurons. J Neurosci., 2020 Oct 12;. doi: 10.1523/JNEUROSCI.1281-19.2020.

Rett syndrome is caused by a deletion or decrease in the amount of a nuclear protein called MeCP2 that binds to methylated DNA.、Highly associated with autism spectrum disorders。On the other hand、It is known that even if the amount of MeCP2 protein is increased, mental developmental disorders occur.。However、Until now, it was not well understood how the amount of MeCP2 causes mental illness.。In this study, the amount of MeCP2 regulates the three-dimensional structure of chromatin in nerve cells.、The possibility of changing the expression of various genes was clearly shown using a mouse model.。This work is based on the research conducted by Lecturer Ishida at Zogbhi Lab while studying abroad.、After returning to Japan, I wrote a paper about it.。

■Artificial synapse-forming molecules is to restore the excitatory synaptic（Science）2020.8.28

Suzuki K†, Elegheert J †, Song I†, Sasakura H †, Senkov O, Matsuda K, Kakegawa W, Clayton AJ, Chang VT, Ferrer Ferrer M, Miura E, Kaushik R,Ikeno M, And Morioka, Takeuchi Y, Shimada T, Otsuka S, Stoyanov S, Watanabe M, Takeuchi K, Dityatev A*, Aricescu AR *, Them so M * (†Co-authored, *Co-responsible author) A synthetic synaptic organizer protein restores glutamatergic neuronal circuits. Science, 369:6507 (2020), Summary article. doi: 10.1126/science.abb4853

The international joint research of structural biology and neurobiologist、Artificial protein CPTX was born with a strong synapse formation force。When the CPTX injected into the living body、In each of the animal model of cerebellar ataxia, Alzheimer's disease, spinal cord injury、Symptoms with synapse formation was found to be dramatically improved。Aricescu Research (UK · MRC-LMB) · Dityatev Institute is a joint research with (Germany · DZNE) · Ken Takeuchi (Japan, Aichi Medical University)。Patent application already。

■Capsaicin-induced mitochondrial injury resistance mechanism in the trigeminal ganglion（Mol Pain）2020.9.28

Shibata M, Kayama Y, Takizawa T, Ibata K, Shimizu T, Yuzaki M, Suzuki N, Nakahara J. Resilience to capsaicin-induced mitochondrial damage in trigeminal ganglion neurons. Mol Pain., 2020 Jan-Dec;16:1744806920960856. doi: 10.1177/1744806920960856

Capsaicin is involved in pain reception in the trigeminal nerve as an agonist of TRPV1。This paper clarified the mechanism of mitochondrial damage induced by frequent administration of capsaicin.。This research is a joint research with Keio University Department of Neurology。Assistant Professor Ibata helped with Ca imaging。

■Observation method of ascending cerebellar morphology using photo-electron correlation microscopy (CLEM)（JEOL News）2020.3.1
Suga M, Nishioka H, Konishi K, Take A, Miura E, Matsuda K, Yuzaki M. Practical Workflow of CLEM – Trace of Climbing FIber in Cerebellar Cortex of Mice. JEOL News, 55:01, 2020.
Abnormalities of synaptic molecules are known to be involved in many developmental disorders and mental disorders。And therefore、Elucidating where and how synaptic molecules are localized in synapses is an extremely important task。To answer this question, we will clarify the localization of the molecule with a high-resolution fluorescence microscope and、Optical-Electron Correlation Microscopy (CLEM) is a powerful tool for reconstructing fine structures with an electron microscope.。there、We are JEOL(Joint research with JEOL) and Nikon。This paper describes the technical workflow。

■In order to nerve cell type-specific Cre driver does not work in the germline（Neuron）2020.2.8
Luo L, …, Yuzaki M, Zoghbi HY, Kawabe H, Craig AM. Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines: Prevalence of Germline Recombination and Influencing Factors. Neuron.. <HTML><HEAD><meta http-equiv="content-type" content="text/html;charset=utf-8"> <TITLE>302 Moved</TITLE></HEAD><BODY> <H1>302 Moved</H1> The document has moved <A HREF="http://ipv4.google.com/sorry/IndexRedirect?continue=http://translate.google.com/translate_a/t?client=a&q=pii&q=S0896-6273&q=j.neuron.2015.05.013&q=elegans+Punctin+Clusters+GABAA+Receptors+via&q=Neuroligin&q=Binding+and+UNC-40%2FDCC+Recruitment&tl=en&sl=auto&amp;q=CGMSBMu9aZ8Y2OjZqwUiGQDxp4NLW_vuiJQA0cSRRi_F4WdisaRqdCQ">here</A>. </BODY></HTML> :S0896-6273(20)30008-8, 2020.[Epub ahead of print]
Is specific Cre driver mouse line to nerve cell types that are available on the world、For an example that would work in the germline、Ann Marie Craig was compiled with the Remedy。We also contains several lines of mouse data。

■Calsyntenin-3 controls the development of excitatory synapses in the hippocampus by binding directly to Nrx（J Biol Chem）2020.1.24
Kim H, Kim D, Kim J, Lee H-Y, Park D, His H, Matsuda K, Sterky FH, Yuzaki M, Kim JY, Choi S-Y, I J, a JW. Calsyntenin-3 directly interacts with neurexins to orchestrate excitatory synapse development in the hippocampus J Biol Chem, 295:9244-9262, 2020. doi: 10.1074/jbc.RA120.013077. Synapse-forming molecules Calsyntenin-3 directly with the particular Neurexin bound to is the job of controlling the mature hippocampal nerve cell formation。It is a joint research with Jaewon Ko our Korea。

■First PET probe to visualize the AMPA receptors in human（Nature Medicine）2020.1.21
Miyazaki T, Nakajima W, Hatano M, Shibata Y, Kuroki Y, Arisawa T, Serizawa A, Sano A, Kogami S, Yamanoue T, Kimura K, Hirata Y, Takada, Ishiwata Y, Sonoda M, Tokunaga M, Seki C, Nagai Y, Minamimoto T, Kawamura K, Zhang M-R, Ikegaya N, Iwasaki M, Kunii N, Kimura Y, Yamashita F, Tags M, Tani H, Nagai N, Koizumi T, Nakajima S, Mimura M, Yuzaki M, Kato H, Higuchi M, Moved H, Takahashi T. Visualization of AMPA receptors in living human brain with positron emission tomography.Nature Medicine, 26:281-288, 2020. doi:10.1038/s41591-019-0723-9.
Thesis of the Yokohama City University School of Medicine Department of Physiology Takahashi and his colleagues came out in Nature Medicine。Keio is part of AMED research is being performed in conjunction with psychiatry, Department of Physiology of the University。